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Image Search Results
Journal: mAbs
Article Title: Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors
doi: 10.4161/19420862.2014.975098
Figure Lengend Snippet: Analyses of the various antibody formats ( A ) To assess the purity and confirm the size of each reagent, the various antibody formats were analyzed by Agilent 2100 bioanalyzer. Proteins were assessed under non-reduced (NR) and reduced conditions (R). 1, IgG; 2, heavy chain; 3, light chain; 4, F(ab)’ 2 ; 5, VH-CH1-hinge; 6, monovalent antibody; 7, hinge-Fc; 8, Fab; 9, light chain and VH-CH1. ( B ) The binding of Hu 15C1 (black circles), monovalent Hu 15C1 (gray triangles), F(ab)’ 2 Hu 15C1 (open circles) and Fab Hu 15C1 (open diamonds) to TLR4 was analyzed by competitive ELISA. To compare the different antibody formats, the same number of binding site was used, i.e., the molar concentration of monovalent and Fab is twice the molar concentration of IgG and F(ab)’ 2 . Results are normalized and expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. ns: not significant.
Article Snippet: Human IgG1, κ, mouse IgG1, κ and
Techniques: Binding Assay, Competitive ELISA, Concentration Assay
Journal: Immunity
Article Title: Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity
doi: 10.1016/j.immuni.2018.12.020
Figure Lengend Snippet:
Article Snippet: mouse IgA, κ Isotype Control, clone
Techniques: Blocking Assay, Negative Control, Recombinant, Staining, Amplification, Enzyme-linked Immunosorbent Assay, PCR Cloning, Software